PDL1 Antibody [6H10]

• Background:

  PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1) . The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3) . Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4) .

• NCBI Gene ID:

  29126

• Swiss Prot:

  Q9NZQ7

• Accession Number:

  NP_054862

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  RF16035

• Clonality:

  Monoclonal

• Clone:

  6H10

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation (Figure 2) shows similar PD-L1 expression profile in both human and mouse cell lines detected by two independent anti-PD-L1 antibodies that recognize different epitopes, RF16035 against the center of human PD-L1 and RF16035 against the extracellular domain. PD-L1 proteins are detected in most of the cell lines, but not in A549 and THP-1 cells by the two independent antibodies. Independent Antibody ValidationRF16035 RF16035 siRNA Knockdown Validation (Figure 3) : Anti-PD-L1 antibody (RF16035) specificity was further verified by PD-L1 specific siRNA knockdown. PD-L1 signal in HeLa cells transfected with PD-L1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA Knockdown ValidationRF16035 Overexpression Validation (Figure 1, 5, 10) : Anti-PD-L1 antibodies (RF16035) can detect the overexpression of PD-L1 protein in 293 cells transfected with PD-L1. Overexpression ValidationRF16035

• Field of Research:

  Immunology

• Purification:

  PD-L1 Antibody is supplied as protein A purified IgG1.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat: (77%), Mouse: (71%)

• Buffer:

  PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PD-L1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG1

• Specificity:

  PD-L1 Antibody has no cross-reactivity to PD-L2.

• Isoform:

  Human PD-L1 has 3 isoforms, including isoform 1 (290aa, 33.3kD), isoform 2 (176aa, 20.2kD), and isoform 3 (178aa, 20.5kD) . This antibody detects human isoform 1&3, mouse and rat PD-L1 (290aa, 33kD for both of them) .

• Symbol:

  CD274

• Positive Control 2:

  Cat. No. 1224 - Daudi Cell Lysate

• Positive Control 3:

  Cat. No. 1340 - Human Stomach Carcinoma Tissue Lysate

• Positive Control 4:

  Cat. No. 1315 - Human Tonsil Tissue

• NCBI Official Name:

  Programmed cell death 1 ligand

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Freeman et al. Exp. Med. 2000; 192:1027-34.

• Background Reference 02:

  Burr et al. Nature 2017; 549:101-5.

• Background Reference 03:

  Mezzadra et al. Nature 2017; 549:106-10.

• Background Reference 04:

  Dong et al. Nat. Med. 1999 5:1365-9.

• Other Product Names:

  PD-L1 Antibody: Programmed cell death 1 ligand-1, programmed death ligand 1, PDL1, PDL-1, B7-H1

• Tested Applications:

  ELISA, WB, IHC-P, ICC, IF

• Protein ID:

  7661534

• Physical Properties:

  Liquid