NKG2D / CD314 Recombinant Protein

• Background:

  NKG2D is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors, also known as KLRK1, CD314, D12S2489E, KLR and killer cell lectin like receptor K1. NKG2D itself forms a homodimer whose ectodomains serve for ligand binding. NKG2D is a major recognition receptor for the detection and elimination of transformed and infected cells as its ligands are induced during cellular stress, either as a result of infection or genomic stress such as in cancer. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.

• NCBI Gene ID:

  22914

• Swiss Prot:

  P26718

• Accession Number:

  NP_031386.2 (T72A)

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by reduced SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  18.5 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 18.5 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Killer cell lectin like receptor K1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Li P, et al. 2001. Nat Immunol. 2 (5) :443-51.

• Background Reference 02:

  Brown MG, et al. 1997. Genomics. 42 (1) :16-25.

• Background Reference 03:

  González S1, et al. Trends Immunol. 2008. 29 (8) :397-403.

• Other Product Names:

  NKG2D, CD314, KLRK1, NK cell receptor D

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3