CD34 Recombinant Protein

• Background:

  CD34 molecule is a cluster of differentiation molecule present on certain cells within the human body. It is a cell surface glycoprotein and functions as a cell-cell adhesion factor. It may also mediate the attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells. As a ~ 110 kDa monomeric cell surface antigen, CD34 is highly glycosylated with nine potential N-linked and numerous potential O-linked glycosylation sites in its extracellular domain. The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia whereas the L-selectin to which it binds is on the T cell. It was indicated that CD34 is a phosphorylation target for activated PKC, and couples to the hematopoietic adapter protein CrkL, which were involved in CD34 signaling pathways. CD34 is abberantly expressed in many kinds of tumors and is implicated in leukemogenesis.

• NCBI Gene ID:

  947

• Swiss Prot:

  P28906

• Accession Number:

  NP_001020280.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Signal Transduction, Stem Cell, Other

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  29.4 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 29.4 kDa. The protein migrates as 67-96 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  CD34molecule

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Simmons DL, et al., 1992, J. Immunol. 148 : 267–71.

• Background Reference 02:

  Satterthwaite AB, et al., 1992, Genomics 12 (4) : 788–94.

• Background Reference 03:

  Nielsen JS, McNagny KM., 2008, J of Cell Science 121 (Pt 22) : 3682–3692.

• Background Reference 04:

  Berg EL, et al., 1998, Am. J. Pathol. 152 (2) : 469–77.

• Other Product Names:

  CD34, RP11-328D5.2

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3