NELL2 Recombinant Protein

• Background:

  NELL2 is a neuron-specific thrombospondin-1-like extracellular protein containing six epidermal growth factor-like domains (1) . It is highly expressed in the hippocampus in vivo (2) and promotes survival of rat primary cultured neurons through activation of JNK and suppression of ERK (3) . As ERK is required for the induction of hippocampal long term potentiation (LTP) and JNK appears to be inhibitory on LTP (4,5), it had been suggested that NELL2 may play a role in synaptic plasticity. More recently, NELL2 has been shown to promote differentiation of motor and sensory neurons and stimulates mitogenesis of dorsal root ganglia in chickens (6) .

• NCBI Gene ID:

  54003

• Swiss Prot:

  Q61220

• Accession Number:

  Q61220

• Tag:

  Fusion Partner:

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Neuroscience

• Purity:

  ~95%

• Buffer:

  100mM sodium phosphate, 10mM Tris, 500mM NaCl, 25 mM imidazole, 2mM MgCl2, 10% gycerol

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

• Calculated Molecular Weight:

  28 kDa (Calculated)

• Applications Notes:

  This recombinant protein can be used for WB and ELISA. For research use only.

• NCBI Official Name:

  NEL-like 2

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Matsuhashi S, Noji S, Koyama E, et al. New gene, nel, encoding a M (r) 93 K protein with EGF-like repeats is strongly expressed in neural tissues of early stage chick embryos. Dev. Dyn. 1995; 203:212-22.

• Background Reference 02:

  Oyasu M, Kuroda S, Nakashita M, et al. Immunocytochemical localization of a neuron-specific thrombospondin-1-like protein, NELL2: light and electron microscopic studies in the rat brain. Mol. Brain Res. 2000; 76:151-60.

• Background Reference 03:

  Aihara K, Kuroda S, Kanamaya N, et al. A neuron-specific EGF family protein, NELL2, promotes survival of neurons through mitogen-activated protein kinases. Mol. Brain Res. 2003; 116:86-93.

• Background Reference 04:

  Rosenblum K, Futter M, Voss K, et al. The role of extracellular kinases I/II in late-phase long-term potentiation. J. Neurosci. 2002; 22:5432-41.

• Other Product Names:

  NELL2 Recombinant Protein: mel91, R75516, A330108N19Rik

• Tested Applications:

  ELISA, WB

• Species:

  Mouse

• Protein ID:

  2494290

• Physical Properties:

  Liquid

• CAS Number:

  9000-83-3