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Sonic Hedgehog Recombinant Protein

CAT: 0223-40-701Size: 5 µgDry Ice: NoHazardous: No
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CAT#:0223-40-701Size:5 µg
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Background
Members of the Hedgehog (Hh) family are highly conserved proteins that are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh), are structurally related, and share a high degree of amino acid sequence identity (e.g. Shh and Ihh are 93% identical) . The biologically active form of each Hh molecule is obtained by autocatalytic cleavage of their precursor proteins, and each corresponds to approximately one half of the N-terminal portion of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can be substituted for one another in experimental systems. Recombinant Murine Sonic Hedgehog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring, chemically modified Cys residue.
CAS Number
9000-83-3
NCBI Gene ID
20423
Swiss Prot
Q62226
Type
Recombinant Proteins
Source
E. coli
Field of Research
Immunology, Neuroscience, Stem Cell, Apoptosis
Purity
≥ 90% by SDS-PAGE gel and HPLC analyses.
Shipping Conditions
Room Temp
Storage Conditions
The recombinant protein is stable for at least 2 years from date of receipt at -20˚ C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚ C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
NCBI Official Name
Sonic hedgehog
NCBI Organism
Mus musculus
Other Product Names
Hhg-1
Species
Mouse
Physical Properties
Lyophilized

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