DKK 1 Recombinant Protein

• Background:

  Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts. DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma. More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via β-catenin mediated Pdgfrα and E-cadherin expression.

• NCBI Gene ID:

  22943

• Swiss Prot:

  O94907

• Accession Number:

  NP_036374.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cell Cycle, Immunology, Signal Transduction

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  26.6 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Dickkopf WNT signaling pathway inhibitor 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Li Y, Lu W, et al., 2010, PLoS One.Jun 8;5 (6) :e11014.

• Background Reference 02:

  Yamaguchi Y, Morita A, et al., 2009, J Investig Dermatol Symp Proc. Aug;14 (1) :73-5.

• Background Reference 03:

  Lieven O, Rüther U.et al., 2011, Dev Biol. Apr 22.

• Other Product Names:

  DKK1, SK

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3