HCoV-HKU1 (isolate N5) S1 Recombinant Protein

• Background:

  It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  85.7 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 85.7 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

• Background Reference 01:

  Wan Y, et al. J Virol. 2020. pii: JVI.00127-20.

• Background Reference 02:

  Benvenuto D, et al. J Med Virol. 2020.

• Background Reference 03:

  Chang CY, et al. AMB Express. 2020. 10 (1) :20.

• Other Product Names:

  Spike, S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1, COVID-19

• Tested Applications:

  WB

• Species:

  HCoV-HKU1 (isolate N5)

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3