HCoV-229E Nucleocapsid Recombinant Protein

• Background:

  Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68) . The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Infectious Disease, COVID-19

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  44.4 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 44.4 kDa. The protein migrates as 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Background Reference 01:

  Reuter M, et al. Virus Genes. 2018. 54:5-16.

• Background Reference 02:

  Grunewald ME, et al. Virology. 2018. 517:62-68.

• Background Reference 03:

  Jeeva S, et al. PLoS One. 2017. 12 (9) :e0184935.

• Other Product Names:

  Nucleocapsid protein, NP, Protein N, COVID-19

• Tested Applications:

  WB

• Species:

  HCoV-229E

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3