IL12 A Recombinant Protein

• Background:

  Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40) . IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. Interleukin-12 subunit alpha (IL12A) also known as NKSF1, CLMF1 and P35, IL12A shows significant sequence similarity to IL-6, G-CSF, and exerts biological activities only when the IL12B is co-expressed. IL12B deficient mice are resistant to the induction of experimental chronic inflammatory diseases whereas IL12A knock-out mice develop more severe forms, suggesting opposite functions of the two subunits in the outcome of chronic inflammatory diseases.

• NCBI Gene ID:

  3592

• Swiss Prot:

  P29459

• Accession Number:

  P29459

• Tag:

  C-Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Immunology, Innate Immunity

• Purity:

  >92% as determined by SDS-PAGE.

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  49.2 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Interleukin 12A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kaliński P, et al., 1997, J. Immunol. 159 : 28–35.

• Background Reference 02:

  Chehimi J, et al., 1993, Eur J Immunol., 23 (8) :1826-30.

• Background Reference 03:

  Thierfelder WE, et al., Nature., 382 (6587) :171-4.

• Other Product Names:

  IL12A, CLMF, IL-12A, NFSK, NKSF1, P35

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3