GDF-2 Recombinant Protein

• Background:

  Human Growth and differentiation factor 2 (GDF-2), also known as Bone morphogenetic protein 9 (BMP-9), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. GDF-2 Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG. ALK1 is a signalling receptor for bone morphogenetic protein-9 (BMP-9) in endothelial cells (ECs) . BMP-9 bound with high affinity to ALK1 and endoglin, and weakly to the type-I receptor ALK2 and to the BMP type-II receptor (BMPR-II) and activin type-II receptor (ActR-II) in transfected COS cells. Binding of BMP-9 to ALK2 was greatly facilitated when BMPR-II or ActR-II were co-expressed.

• Tag:

  Tag Free

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  ACN, TFA in Water

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  12.1 kDa

• Applications Notes:

  This protein carries no "tag". The protein has a calculated MW of 12.1 kDa. The protein migrates as 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Background Reference 01:

  Majumdar M K , Wang E , Morris E A . 2001, 189 (3) :275-84.

• Background Reference 02:

  Scharpfenecker M , Van Dinther M , Liu Z , et al. 2007, 120 (6) :964.

• Background Reference 03:

  Brown, M. A . Journal of Biological Chemistry, 2005, 280 (26) :25111-8.

• Other Product Names:

  GDF-2, GDF2, BMP-9, BMP9

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3