TIM-3 / HAVCR2 Recombinant Protein

• Background:

  Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1) -mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

• Accession Number:

  EHH54703.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Immunology

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  21.6 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.6 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Background Reference 01:

  Monney, L. et al., 2002, Nature, 415:536-541.

• Background Reference 02:

  Sabatos, CA. et al., 2003, Nat. Immunol., 4:1102-1110.

• Background Reference 03:

  Anderson, AC. et al., 2006, Curr. Opin. Immunol., 18:665-669.

• Other Product Names:

  HAVCR2, TIM3, TIMD3, FLJ14428, KIM3

• Tested Applications:

  ELISA, WB

• Species:

  Cynomolgus monkey

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3