GDF-15 / MIC-1 Recombinant Protein

• Background:

  Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1) . Expression of MIC-1 mRNA in monocytoid cells is up-regulated by a variety of stimuli associated with activation, including interleukin 1β, tumor necrosis factor α (TNF-α), interleukin 2, and macrophage colony-stimulating factor but not interferon γ, or lipopolysaccharide (LPS) . It is highly expressed in cardiomyocytes, adipocytes, macrophages, endothelial cells, and vascular smooth muscle cells in normal and pathological condition. GDF-15 increases during tissue injury and inflammatory states and is associated with cardiometabolic risk. Increased GDF-15 levels are associated with cardiovascular diseases such as hypertrophy, heart failure, atherosclerosis, endothelial dysfunction, obesity, insulin resistance, diabetes, and chronic kidney diseases in diabetes. Increased GDF-15 level is linked with the progression and prognosis of the disease condition.

• NCBI Gene ID:

  9518

• Swiss Prot:

  Q99988

• Accession Number:

  NP_004855.2

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Purity:

  >90% as determined by reduced SDS-PAGE.

• Buffer:

  60% ACN, 0.085% TFA

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  14.2 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 14.2 kDa. the protein migrates as 16-17 kDa under reducing (R) condition, and 27 kDa under non-reducing (NR) condition (SDS-PAGE) .

• NCBI Official Name:

  Growthdifferentiationfactor15

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Bootcov MR., et al. 1997, Proc Natl Acad Sci U S A., 94 (21), 11514-9.

• Background Reference 02:

  Adela R., et al. 2015, J Diabetes Res., 2015, 490842.

• Other Product Names:

  GDF15, GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB, NRG-1

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3