GDF-15/MIC-1

• Description:

  GDF-15 belongs to the TGF-β cytokine family whose members play an important role during prenatal development and postnatal growth, remodeling and maintenance of a variety of tissues and organs. GDF-15 is expressed predominantly in placenta and to a much lesser extent in various other tissues. The presence of GDF-15 in amniotic fluid and its elevated levels in the sera of pregnant women suggest a role for GDF-15 in gestation and embryonic development. GDF-15 generally exerts tumor suppressive activities and is one of the predominant factors produced and secreted in response to activation of the p53 pathway. Interestingly, the serum level of GDF-15 is positively correlated with neoplastic progression of several tumor types, including certain colorectal, pancreatic, and prostate cancers. Human GDF-15/MIC-1 is a disulfide linked homodimeric protein consisting of two 114 amino-acid polypeptide chains.

• Synonyms:

  GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15

• NCBI Gene ID:

  9518

• UniProt:

  Q99989

• Accession Number:

  NP_004855.3

• Accession Number mRNA:

  NM_004865

• Chromosomal Location:

  19p13.11

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  ARNGDHCPLG PGRCCRLHTV RASLEDLGWA DWVLSPREVQ VTMCIGACPS QFRAANMHAQ IKTSLHRLKP DTVPAPCCVP ASYNPMVLIQ KTDTGVSLQT YDDLLAKDCH CI

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by a cell inhibition assay using DU-145 cells. The expected ED50 for this effect is 1.0-2.0 μg/ml.

• Length:

  114

• Form:

  Lyophilized

• Molecular Weight:

  24.6 kDa

• Host or Source:

  CHO cells