NOTCH2 Recombinant Protein

• Background:

  Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus . Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity) . Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation . Positively regulates self-renewal of liver cancer cells .

• NCBI Gene ID:

  4853

• Swiss Prot:

  Q04721

• Accession Number:

  NP_077719.2

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  80.5 kDa

• Applications Notes:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 80.5 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Notchreceptor2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Michael A Simpson, et. al., 2011, Nat Genet.6;43 (4) :303-5.

• Background Reference 02:

  Isidor B, et. al., 2011, Nat. Genet. 43:306-308.

• Background Reference 03:

  Fukushima H, et. al., 2017, Mol. Cell. 68:645-658.

• Background Reference 04:

  Zhu PNat, et. al., 2015, Commun. 6:7122-7122.

• Other Product Names:

  NOTCH2, hN2, N2ECD

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3