ErbB3 / Her3 Recombinant Protein

• Background:

  ErbB3, also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

• NCBI Gene ID:

  2065

• Swiss Prot:

  P21860

• Accession Number:

  NP_001973.2

• Tag:

  Strep Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cancer

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  71.7 kDa

• Applications Notes:

  This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 71.7 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Erb-b2 receptor tyrosine kinase 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Singer, E. etal., 2001, J. Biol. Chem. 276 (47) : 44266–74

• Background Reference 02:

  Kraus, M.H.etal.,1989, Proc.Natl.Acad.Sci.86:9193-9197.

• Background Reference 03:

  Carraway, K.L.etal.,1994, J.Biol.Chem.269:14303-14306.

• Background Reference 04:

  Wallasch, C.etal.,1995, EMBO.J.14:4267-4275.

• Other Product Names:

  ERBB3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3