CD27 Ligand / CD70 Recombinant Protein

• Background:

  Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.

• NCBI Gene ID:

  21948

• Swiss Prot:

  O55237

• Accession Number:

  NP_035747.1

• Tag:

  Mouse IgG Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Immunology

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  50 mM Tris, 100 mM Glycine, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  43.5 kDa

• Applications Notes:

  This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 43.5 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  CD70 antigen

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  McEarchern JA. et al., 2008, Clin Cancer Res., 14 (23) :7763-72.

• Background Reference 02:

  Croft M., 2003, Nat Rev Immunol., 3 (8) :609-20.

• Background Reference 03:

  Kobata, TS. et al., 1995, Proc. Natl. Acad. Sci. USA, 92: 11249–11253.

• Background Reference 04:

  Raman V.S. et al., 2003, J Immunol., 171 (11) :5876-81.

• Other Product Names:

  CD70, CD27LG, TNFSF7, TNFSF7G, CD27L

• Tested Applications:

  ELISA, WB

• Species:

  Mouse

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3