Cathepsin B Recombinant Protein

• Background:

  Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L) ; In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.

• NCBI Gene ID:

  1508

• Swiss Prot:

  P07858

• Accession Number:

  NP_001899

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cancer, Neuroscience, Signal Transduction

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  50 mM Tris, 150 mM NaCl, pH8.0

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  36.7 kDa (pro-form) and 29 kDa (mature-form)

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Cathepsin B

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Basrur V., et al., 2003, J. Proteome Res. 2:69-79.

• Background Reference 02:

  Chi A., et al., 2006, J. Proteome Res. 5:3135-3144.

• Background Reference 03:

  Royer-Zemmour B., et al., 2008, Hum. Mol. Genet. 17:3617-3630.

• Background Reference 04:

  Cao L., et al., 1994, Gene 139 (2) : 163–9.

• Other Product Names:

  CTSB, CPSB, APPS

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3