BACE1 Recombinant Protein

• Background:

  Beta-secretase 1 (BACE1) is also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP) . Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD.

• NCBI Gene ID:

  23621

• Swiss Prot:

  P56817

• Accession Number:

  NP_036236.1

• Tag:

  N-Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cancer, Neuroscience, Other

• Purity:

  >85% as determined by SDS-PAGE.

• Buffer:

  50 mM tris, 100 mM glycine, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  75 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Beta-site APP-cleaving enzyme 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Willem M, et al., 2006, Science 314 (5799) : 664–6.

• Background Reference 02:

  Zacchetti D, et al., 2007, Neurodegener Dis. 4: 117-126.

• Background Reference 03:

  Zhao J. et al., 2007, J. Neurosci. 27: 3639- 3649.

• Other Product Names:

  BACE1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149, Memapsin-2

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3