ApolipoRecombinant Protein E / APOE Recombinant Protein

• Background:

  APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL) . It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles.

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, 5 mM CHAPS, 2 mM DTT, pH 7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  36.1 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.1 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Background Reference 01:

  Arnon R, et al. 1991. Biochim Biophys Acta. 1085 (3) :336-42.

• Background Reference 02:

  Ji ZS, et al. 1997. J Biol Chem. 272 (50) :31285-92.

• Background Reference 03:

  Marcel YL, et al. 1983. Biochim Biophys Acta. 750 (2) :411-7.

• Other Product Names:

  Apolipoprotein E, APOE, Apo-E

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3