Apo A1 Recombinant Protein

• Background:

  ApoA1 is also known as apolipoprotein A-I, ApoA-I , and is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Chylomicrons secreted from the intestinal enterocyte also contain ApoA1 but it is quickly transferred to HDL in the bloodstream. The protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. In addition, it has been shown that ApoA1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin.

• NCBI Gene ID:

  335

• Swiss Prot:

  P02647

• Accession Number:

  NP_000030.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cancer, Cell Cycle, Homeostasis, Other

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  29 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Apolipoprotein A-I

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wasan KM , et al. 2008, Nature Reviews Drug Discovery 7 : 84–99.

• Background Reference 02:

  Yui Y, et al. 1988, J. Clin. Invest. 82 (3) : 803–7.

• Background Reference 03:

  Eggerman, T.L. et al., 1991, J. Lipid Res. 32: 821-828.

• Background Reference 04:

  Voyiaziakis, E. et al., 1998. J. Lipid Res. 39: 313-321.

• Other Product Names:

  Apolipoprotein A-I, APOA1, MGC117399

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3