Alkaline Phosphatase Recombinant Protein

• Background:

  Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) is a cell membrane protein which belongs to the alkaline phosphatase family. There are at least four distinct but related alkaline phosphatases in humans: intestinal AP (IAP), placental AP (PLAP), germ cell AP (GCAP) and their genes are clustered on chromosome 2, tissue-nonspecific isozyme (TNAP) which gene is located on chromosome 1. Alkaline phosphatases (APs) are dimeric enzymes, it catalyze the hydrolysis of phosphomonoesters with release of inorganic phosphate. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. This isozyme may play a role in skeletal mineralization. Mutations in ALPL gene have been linked directly to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones, and this disorder can vary depending on the specific mutation since this determines age of onset and severity of symptoms.

• NCBI Gene ID:

  249

• Swiss Prot:

  P05186

• Accession Number:

  P05186

• Tag:

  C-6 His tag

• Type:

  Recombinant Proteins

• Source:

  Human Cells

• Field of Research:

  Other

• Buffer:

  Supplied as a 0.2 um filtered solution of 20mM HEPES, 150mM NaCl, 2mM MgSO4, 0.1mM ZnCl2, pH 7.5. It is not recommended to reconstitute to a concentration less than 100 ug/ml.

• Shipping Conditions:

  Dry Ice

• Calculated Molecular Weight:

  54.5 kD

• Applications Notes:

  This recombinant protein can be used for biological assays. For research use only.

• NCBI Official Name:

  Alkaline phosphatase, liver/bone/kidney

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Alkaline Phosphatase, Tissue-Nonspecific Isozyme, AP-TNAP, TNSALP, Alkaline Phosphatase Liver/Bone/Kidney Isozyme, ALPL

• Species:

  Human

• Protein ID:

  578798860

• Physical Properties:

  Liquid

• CAS Number:

  9000-83-3