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CD47 Antibody (Biotin)

CAT: 0013-GTR18195121Size: 0.5 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18195121Size:0.5 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse monoclonal antibody against CD47 conjugated to Biotin
Product Name Alternative
Anti-Antigenic Surface Determinant Protein OA3 antibody, anti-IAP antibody, anti-Integrin Associated Protein antibody, anti-Integrin Associated Signal Transducer antibody, anti-MER6 antibody, anti-OA3 antibody, anti-Protein MER6 antibody, anti-Rh Related Antigen antibody
UniProt
Q08722
Reactivity
Human
Immunogen
A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)
Target
CD47
Clonality
Monoclonal
Clone
CD47/3019
Conjugation
Biotin
Field of Research
Immunology & Inflammation; Cell Biology
Purification
Protein G
Concentration
Purified Ab conjugated to Biotin
Dilution
Immunofluorescence (2-4ug/ml) ; Flow Cytometry (5ul per million cells) ; , Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Form
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Molecular Weight
~50kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: MCF-7, HUVEC or OVCA3 cells (IF/FACS) . Human placenta, brain or ovarian tumors (IHC) .
Tested Applications
ELISA, FC, IF, IHC
Host or Source
Mouse
Preservative
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Isotype
IgG1
Entrez
961

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