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GDF15 Antibody

CAT: 0013-GTR18187357Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18187357Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NAG-1 antibody
Product Name Alternative
Rat anti-NAG1 antibody, NAG-1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor
UniProt
Q99988
Reactivity
Human, Mouse
Immunogen
This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to C-term mouse NAG-1 protein. Cross reactivity to MBP is negative.
Target
GDF15
Clonality
Monoclonal
Clone
6D12.H10.E4
Conjugation
Unconjugated
Concentration
1.09 mg/mL
Dilution
ELISA: 1:2,000, WB: 1:1,000
Purity
This product was purified from concentrated tissue culture supernatant Protein G chromatography. This antibody reacts with the C-terminus of endogenous NAG-1 protein from mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This Protein G Anti-NAG1 purified antibody has been tested by ELISA, SDS-PAGE, and western blot for mouse NAG-1 protein. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately ~58kDa using the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
Host or Source
Rat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG2a

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