GOT1 Antibody (HRP)

CAT: 0013-GTR18184125Size: 25 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18184125Size:25 µL

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Description

Aspartate Transaminase antibody (Peroxidase)

Product Name Alternative

Sheep anti-Aspartate Transaminase HRP conjugated Antibody, peroxidase conjugated sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody

UniProt

P00503

Reactivity

Porcine

Immunogen

Aspartate Aminotransferase (AST) [Pig Heart]

Target

GOT1

Clonality

Polyclonal

Conjugation

HRP

Concentration

1 mg/mL

Dilution

ELISA: 1:10,000 - 1:40,000, WB: 1:1,000 - 1:5,000

Purity

Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart]. Cross reactivity against Aspartate Aminotransferase (AST) from other sources may occur but has not been specifically determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Aspartate Transaminase Peroxidase Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 ug of Aspartate Aminotransferase (AST) [Pig Heart] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Tested Applications

DOT, ELISA, WB

NCBI Accession Number

NP_999092.1

Host or Source

Sheep

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P00503

Aspartate aminotransferase, cytoplasmic

AATC_PIG · Sus scrofa

View on UniProt ↗

Primary accession: P00503
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: GOT1
Protein existence: 1: Evidence at protein level
Organism: Sus scrofa (Pig)
Taxonomy ID: 9823
Alternative names: cAspAT
EC number: 2.6.1.1, 2.6.1.3
Processing: —
Secondary accessions: —

Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Biological process

Amino-acid biosynthesis

Molecular function

AminotransferaseTransferase

Cellular component

Cytoplasm

Ligand

Pyridoxal phosphate

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