Products for Research Use Only

AmpC Antibody (HRP)

CAT: 0013-GTR18183996Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18183996Size:100 µg
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Description
Penicillinase antibody (Peroxidase)
Product Name Alternative
Rabbit anti-Penicillinase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Penicillinase Antibody, Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody, Cephalosporinase
UniProt
P05364
Reactivity
Bacteria
Immunogen
Penicillinase [Enterobacter cloacae]
Target
AmpC
Clonality
Polyclonal
Conjugation
HRP
Concentration
1.0 mg/mL
Dilution
IP: 1:100, WB: 1:250
Purity
Penicillinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae]. Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Penicillinase Peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Penicillinase [Enterobacter cloacae] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Tested Applications
DOT, IP, WB
NCBI Accession Number
AAO24600.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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