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AmpC Antibody

CAT: 0013-GTR18183912Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183912Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Penicillinase antibody
Product Name Alternative
Rabbit anti-Penicillinase Antibody, Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody, Cephalosporinase
UniProt
P05364
Reactivity
Bacteria
Immunogen
Penicillinase [E.cloacae]
Target
AmpC
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/ml
Dilution
IP: 1:100, WB: 1:500 - 1:5,000
Purity
Anti-Penicillinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Penicillinase [E.cloacae]. Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Penicillinase Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Penicillinase [E.cloacae] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:21,000 of the reconstitution concentration is suggested for this product.
Tested Applications
IP, WB
NCBI Accession Number
AAO24600.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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