AmpC Antibody (Biotin)

CAT: 0013-GTR18184073Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18184073Size:100 µg

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Description

Penicillinase antibody (Biotin)

Product Name Alternative

Rabbit anti-Penicillinase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Penicillinase Antibody, Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody, Cephalosporinase

UniProt

P05364

Reactivity

Bacteria

Immunogen

Penicillinase [Enterobacter cloacae]

Target

AmpC

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1.0 mg/mL

Dilution

IP: 1:100, WB: 1:500 - 1:2,000

Purity

Anti-Penicillinase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae]. Cross reactivity against Penicillinase from other sources is unknown.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Penicillinase Biotin Conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 100 μL

Tested Applications

DOT, IP, WB

NCBI Accession Number

AAO24600.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P05364

Beta-lactamase

AMPC_ENTCL · Enterobacter cloacae

View on UniProt ↗

Primary accession: P05364
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: ampC
Protein existence: 1: Evidence at protein level
Organism: Enterobacter cloacae
Taxonomy ID: 550
Alternative names: —
EC number: 3.5.2.6
Processing: Precursor
Secondary accessions: —

Protein keywords

Technical term

3D-structure

Biological process

Antibiotic resistance

Molecular function

Hydrolase

Cellular component

Periplasm

Domain

Signal

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