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GlnA Antibody (HRP)

CAT: 0013-GTR18183533Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183533Size:25 µL
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Description
Glutamine Synthetase antibody (Peroxidase)
Product Name Alternative
Goat anti-Glutamine Synthetase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase
UniProt
Q79VE3
Reactivity
Bacteria
Immunogen
Glutamine Synthetase [Microbial]
Target
GlnA
Clonality
Polyclonal
Conjugation
HRP
Concentration
1.0 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:2,500
Purity
Glutamine Synthetase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Glutamine Synthetase has been assayed against 1.0 ug of Glutamine Synthetase [Microbial] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
CAG33988.1
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · Q79VE3

Glutamine synthetase

Q79VE3_CORGL · Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534)

View on UniProt ↗
Primary accession
Q79VE3
Review status
UniProtKB unreviewed (TrEMBL)
Gene
—
Protein existence
3: Inferred from homology
Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534)
Taxonomy ID
196627
Alternative names
—
EC number
6.3.1.2
Processing
—
Secondary accessions
O32354
Protein keywords

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Cellular component

Cytoplasm

Molecular function

Ligase

PTM

Phosphoprotein

Technical term

Reference proteome

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