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GlnA Antibody

CAT: 0013-GTR18183470Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183470Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Glutamine Synthetase antibody
Product Name Alternative
Goat anti-Glutamine Synthetase Antibody, Glutamine synthetase, GS
UniProt
Q79VE3
Reactivity
Bacteria
Immunogen
Glutamine Synthetase [native from brevibacterium species]
Target
GlnA
Clonality
Polyclonal
Conjugation
Unconjugated
Dilution
ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
Purity
Anti-Glutamine Synthetase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Glutamine Synthetase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Glutamine Synthetase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product. It is suitable for Immunohistochemistry also. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
CAG33988.1
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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