Protein Blocking Reagent (animal serum free)

Protein Blocking Reagent (animal serum free)

• Description: This protein blocking solution does not contain any phosphate, therefore it is good for alkaline phosphatase assays. It does not contain azide therefore peroxidase is not inhibited. It does not contain any animal serum; it can be used for all IHC, ICC kits, ELISA and WB
• Applications: WB, IHC, ICC, ELISA
• Assay Principle: IHC/ICC procedure for frozen sections, paraffin sections and cell smears. 1. Deparafinize and hydrate tissue sections through xylene or other clearing agents and graded alcohols.(For frozen sections or cell smears; use unfixed, acetone fixed or appropriate fixative for the antigen in question; for cell smears it may be necessary to permealize the cell by detergent, please refer to antibody protocol) 2. Wash 2-3 with distilled or deionized water. 3. Incubate sections/cell smear in Endoblocker (#1) for 5-10 minutes at room temperature or 37°C. Note: If antigen retriever is required it can be applied after this stage. 4. Wash slide with PBS or Tris saline (with 0.02-0.05% nonionic detergent, Triton X100, Tween 20 or NP-40) or washing buffer (Immuno Automation buffer IBSC cat # AR-6561) 3-5X 5. Incubate sections/ cell smear in Protein blocking solution, for 10 minutes. at RT or 37ºC 6. Wash slide with PBS 1X. 7. Follow instructions for IHC/ICC.