Pepsin Reagent (ready to use)

CAT:

246-AR-6543-02

Size:

100 mL

Price:

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Description: Pepsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
Applications: IHC
Assay Principle: 1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer. 2. Remove buffer, add 2-4 drops of Pepsin reagent to cover tissue section. 3. Incubate at 37oC or at 56oC for 5-10 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-10 minutes are sufficient at the above temperatures. In some cases it may require 15 minute.). 4. Discard Pepsin reagent, wash 3-5 times with buffer. 5. The tissue sections are ready for further IHC protocol. NOT RECOMMENDED FOR CYTOKERATIN (clone: AE3) ANTIBODY
Shipping Conditions: Blue Ice
Storage Conditions: 4°C

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