CKIP-1 Antibody (N-term) [APR15459G]

• Background:

  Tissue Location: Abundantly expressed in skeletal muscle and heart, moderately in kidney, liver, brain and placenta and sparingly in the pancreas and lung. Easily detectable in cell lines such as MOLT-4, HEK293 and Jurkat.

• Synonyms:

  Pleckstrin homology domain-containing family O member 1, PH domain-containing family O member 1, C-Jun-binding protein, JBP, Casein kinase 2-interacting protein 1, CK2-interacting protein 1, CKIP-1, Osteoclast maturation-associated gene 120 protein, PLEKHO1, CKIP1, OC120

• Gene Name:

  PLEKHO1

• Gene ID:

  51177

• UniProt:

  Q53GL0

• Chromosomal Location:

  Cell membrane; Peripheral membrane protein. Nucleus. Cytoplasm Note=Predominantly localized to the plasma membrane through the binding to phosphatidylinositol 3-phosphate (PubMed:14729969) . In C2C12 cells, with the absence of growth factor, it is found in the nucleus (PubMed:14729969) . It rapidly translocates to the plasma membrane after insulin stimulation (PubMed:14729969) . In response to TNF, it translocates from the plasma membrane to the cytoplasm and then to the nucleus accompanied by cleavage by caspase-3 (PubMed:15706351) However, the subcellular location is highly dependent of the cell type, and this explains why it is found exclusively at the plasma membrane, in some type of cells (Probable) . {ECO:0000269|PubMed:14729969, ECO:0000269|PubMed:15706351, ECO:0000305}

• Applications:

  WB, IHC-P, FC

• Dilution:

  WB--1:1000 IHC-P--1:100~500 FC--1:10~50

• Form:

  Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

• Function:

  Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP) . May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2) . Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.

• Molecular Weight:

  46237

• Storage Conditions:

  Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

• Overview:

  We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CKIP-1 Antibody (N-term) [APR15459G].

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/?term=51177

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q53GL0