Anti-Hu Ig Lambda Light Chain Alexa Fluor® 700

• Background:

  Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

• Specifications:

  The mouse monoclonal antibody 1-155-2 recognizes lambda light chains (22.5 kDa) of human immunoglobulin.

• Certification:

  RUO

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human IgA1 lambda myeloma protein

• Target Antigen:

  Ig Lambda Light Chain

• Isotype:

  Igg1 κ

• Clone:

  1-155-2

• Applications:

  Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests. Extracellular and intracellular staining.

• Validated Applications:

  FC

• Format:

  Alexa Fluor® 700

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Kubagawa H, Gathings WE, Levitt D, Kearney JF, Cooper MD: Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence. J Clin Immunol. 1982 Oct2(4):264-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/6815220,*Bahler DW, Pindzola JA, Swerdlow SH: Splenic marginal zone lymphomas appear to originate from different B cell types. Am J Pathol. 2002 Jul161(1):81-8., URL: https://pubmed.ncbi.nlm.nih.gov/12107092/,*Tierens AM, Holte H, Warsame A, Ikonomou IM, Wang J, Chan WC, Delabie J: Low levels of monoclonal small B cells in the bone marrow of patients with diffuse large B-cell lymphoma of activated B-cell type but not of germinal center B-cell type. Haematologica. 2010 Aug95(8):1334-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/20145271,

• Other References:

  *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690?ordinalp,

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• CAS Number:

  9007-83-4