Anti-MICA/MICB PE

• Description:

  MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. Binding of MICA/MICB receptor, the NKG2D, leads to cytolytic response of NK cells, Tc cells, and gamma/delta T cells. Alternative splicing results in multiple isoforms, and some of them have been associated with susceptibility to psoriasis and psoriatic arthritis. Shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.

• Certification:

  RUO

• Reactivity:

  Human

• Immunogen:

  Transfected C1R cells expressing MICA

• Target Antigen:

  MICA/MICB

• Clone:

  6D4

• Applications:

  FC (QC tested)

• Concentration:

  0.1 mg/mL

• Format:

  PE

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Ghadially H, Brown L, Lloyd C, Lewis L, Lewis A, Dillon J, Sainson R, Jovanovic J, Tigue NJ, Bannister D, Bamber L, Valge-Archer V, Wilkinson RW: MHC class I chain-related protein A and B (MICA and MICB) are predominantly expressed intracellularly in tumour and normal tissue. Br J Cancer. 2017 Apr 25;116 (9) :1208-1217., URL: https://pubmed.ncbi.nlm.nih.gov/28334733/, *Ritter C, Fan K, Paulson KG, Nghiem P, Schrama D, Becker JC: Reversal of epigenetic silencing of MHC class I chain-related protein A and B improves immune recognition of Merkel cell carcinoma. Sci Rep. 2016 Feb 23;6:21678. doi: 10.1038/srep21678., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=ritter+c%2C+fan+k%2C+paulson+kg , *Del Toro-Arreola S, Arreygue-Garcia N, Aguilar-Lemarroy A, Cid-Arregui A, Jimenez-Perez M, Haramati J, Barros-Nuñ ez P, Gonzalez-Ramella O, Del Toro-Arreola A, Ortiz-Lazareno P, Hernandez-Flores G, Bravo-Cuellar A, Daneri-Navarro A, Jave-Suarez LF: MHC class I-related chain A and B ligands are differentially expressed in human cervical cancer cell lines. Cancer Cell Int. 2011 Jun 1;11:15. doi: 10.1186/1475-2867-11-15., URL: https://pubmed.ncbi.nlm.nih.gov/21631944/, *Carreno BM, Garbow JR, Kolar GR, Jackson EN, Engelbach JA, Becker-Hapak M, Carayannopoulos LN, Piwnica-Worms D, Linette GP: Immunodeficient mouse strains display marked variability in growth of human melanoma lung metastases. Clin Cancer Res. 2009 May 15;15 (10) :3277-86., URL: https://pubmed.ncbi.nlm.nih.gov/19447870/, *Sester M, Koebernick K, Owen D, Ao M, Bromberg Y, May E, Stock E, Andrews L, Groh V, Spies T, Steinle A, Menz B, Burgert HG: Conserved amino acids within the adenovirus 2 E3/19K protein differentially affect downregulation of MHC class I and MICA/B proteins. J Immunol. 2010 Jan 1;184 (1) :255-67., URL: https://www.ncbi.nlm.nih.gov/pubmed/19949079 , *Xu X, Rao G, Gaffud MJ, Ding HG, Maki G, Klingemann HG, Groh V, Spies T, Caillat-Zucman S, Gattuso P, Plate J, Prinz RA: Clinicopathological significance of major histocompatibility complex class I-related chain a and B expression in thyroid cancer. J Clin Endocrinol Metab. 2006 Jul;91 (7) :2704-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/16621903 , *Steinle A, Li P, Morris DL, Groh V, Lanier LL, Strong RK, Spies T: Interactions of human NKG2D with its ligands MICA, MICB, and homologs of the mouse RAE-1 protein family. Immunogenetics. 2001 May-Jun;53 (4) :279-87., URL: https://www.ncbi.nlm.nih.gov/pubmed/11491531 , *Groh V, Rhinehart R, Secrist H, Bauer S, Grabstein KH, Spies T: Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB. Proc Natl Acad Sci U S A. 1999 Jun 8;96 (12) :6879-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=broad+tumor-associated+expression+and+recognition+by+tumor-derived , *Groh V, Bahram S, Bauer S, Herman A, Beauchamp M, Spies T: Cell stress-regulated human major histocompatibility complex class I gene expressed in gastrointestinal epithelium. Proc Natl Acad Sci U S A. 1996 Oct 29;93 (22) :12445-50. , URL: https://www.ncbi.nlm.nih.gov/pubmed/8901601

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 6D4 recognizes a common extracellular epitope on MICA and MICB glycoproteins, transmembrane ligands of CD314 (NKG2D), and is able to block CD314-mediated activation of NK cells and cytotoxic T cells.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-5 μg/ml.

• Isotype:

  Mouse IgG2a