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Anti-ARHGEF4 PE

CAT: 0270-1P-723-C100Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0270-1P-723-C100Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli – stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC) -binding region (ABR) it contains Dbl homology (DH), pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.
Certification
RUO
Reactivity
Human
Immunogen
Recombinant fragment of human ARHGEF4 (amino acids 143-271)
Target Antigen
ARHGEF4
Clone
ARHGEF-08
Applications
FC-IC (QC tested)
Concentration
0.1 mg/mL
Format
PE
Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Other References
*Zhang Z, Chen L, Gao L, Lin K, Zhu L, Lu Y, Shi X, Gao Y, Zhou J, Xu P, Zhang J, Wu G: Structural basis for the recognition of Asef by adenomatous polyposis coli. Cell Res. 2012 Feb;22 (2) :372-86., URL: http://www.ncbi.nlm.nih.gov/pubmed/21788986, , *Lyons R, Williams O, Morrow M, Sebire N, Hubank M, Anderson J: The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL-AML1 to promote leukaemic transformation. Leuk Res. 2010 Jan;34 (1) :109-15., URL: https://pubmed.ncbi.nlm.nih.gov/19628279/, *Kawasaki Y, Tsuji S, Sagara M, Echizen K, Shibata Y, Akiyama T: Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3-kinase. J Biol Chem. 2009 Aug 14;284 (33) :22436-43., URL: http://www.ncbi.nlm.nih.gov/pubmed/19525225
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Specificity
The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. The epitope is located in the rough at the region of SH3 domain.
Applications Notes
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Isotype
Mouse IgG1

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