Anti-Hu CD255 PE

CD255 / TWEAK (TNF-related weak inducer of apoptosis), a type II transmembrane protein expressed as membrane-bound and secreted form, can induce apoptosis in many tissues and cell lines through its receptor CD266 / TWEAK R. On the other hand, in endothelial cells this interaction can induce proliferation and promote angiogenesis including neovascularization of tumours. CD255 can act in a juxtacrine manner to initiate cellular responses, and induces secretion of pro-inflammatory cytokines. Besides CD266, CD255 may also bind to DR3.

Human

Human CD255-transfected 2PK-3 cells

CD255

CARL-1

FC (QC tested)

PE

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Yoriki R, Akashi S, Sho M, Nomi T, Yamato I, Hotta K, Takayama T, Matsumoto S, Wakatsuki K, Migita K, Yagita H, Nakajima Y: Therapeutic potential of the TWEAK/Fn14 pathway in intractable gastrointestinal cancer. Exp Ther Med. 2011 Jan;2 (1) :103-108, URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=yoriki+r+akashi+s , *Nakayama M, Ishidoh K, Kojima Y, Harada N, Kominami E, Okumura K, Yagita H: Fibroblast growth factor-inducible 14 mediates multiple pathways of TWEAK-induced cell death. J Immunol. 2003 Jan 1;170 (1) :341-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/12496418 , *Hosokawa Y, Hosokawa I, Ozaki K, Nakae H, Matsuo T: Proinflammatory effects of tumour necrosis factor-like weak inducer of apoptosis (TWEAK) on human gingival fibroblasts. Clin Exp Immunol. 2006 Dec;146 (3) :540-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/17100776 , *Saitoh T, Nakayama M, Nakano H, Yagita H, Yamamoto N, Yamaoka S: TWEAK induces NF-kappaB2 p100 processing and long lasting NF-kappaB activation. J Biol Chem. 2003 Sep 19;278 (38) :36005-12., URL: http://www.ncbi.nlm.nih.gov/pubmed/12840022 , *Brown SA, Ghosh A, Winkles JA: Full-length, membrane-anchored TWEAK can function as a juxtacrine signaling molecule and activate the NF-kappaB pathway. J Biol Chem. 2010 Jun 4;285 (23) :17432-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/20385556, *Nakayama M, Kayagaki N, Yamaguchi N, Okumura K, Yagita H: Involvement of TWEAK in interferon gamma-stimulated monocyte cytotoxicity. J Exp Med. 2000 Nov 6;192 (9) :1373-80., URL: https://www.ncbi.nlm.nih.gov/pubmed/11067885

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody CARL-1 recognizes an extracellular epitope of CD255 / TWEAK, a type II transmembrane protein of the TNF superfamily able to induce apoptosis weakly in many cell types.

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