Anti-Ms CD105 PE

• Description:

  CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

• Certification:

  RUO

• Reactivity:

  Mouse

• Immunogen:

  Inflamed mouse skin

• Target Antigen:

  CD105

• Clone:

  MJ7/18

• Applications:

  FC (QC tested)

• Concentration:

  0.5 mg/mL

• Format:

  PE

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Ge AZ, Butcher EC: Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand. Gene. 1994 Jan 28;138 (1-2) :201-6., URL: https://pubmed.ncbi.nlm.nih.gov/8125301/, *Herouet C, Cottin M, LeClaire J, Enk A, Rousset F: Contact sensitizers specifically increase MHC class II expression on murine immature dendritic cells. In Vitr Mol Toxicol. 2000 Summer;13 (2) :113-23., URL: http://www.ncbi.nlm.nih.gov/pubmed/11031322 , *Dassler K, Roohi F, Lohrke J, Ide A, Remmele S, Hütter J, Pietsch H, Pison U, Schütz G: Current limitations of molecular magnetic resonance imaging for tumors as evaluated with high-relaxivity CD105-specific iron oxide nanoparticles. Invest Radiol. 2012 Jul;47 (7) :383-91, URL: http://www.ncbi.nlm.nih.gov/pubmed/22659596 , *Rosu-Myles M, She YM, Fair J, Muradia G, Mehic J, Menendez P, Prasad SS, Cyr TD: Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells. PLoS One. 2012;7 (6) :e38954. doi: 10.1371/journal.pone.0038954., URL: https://pubmed.ncbi.nlm.nih.gov/22719999/, *Cho SK, Bourdeau A, Letarte M, Zúñ iga-Pflücker JC: Expression and function of CD105 during the onset of hematopoiesis from Flk1 (+) precursors. Blood. 2001 Dec 15;98 (13) :3635-42., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=cho+sk+bourdeau+a , *Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun;25 (3B) :1851-64., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=warrington+k+hillarby+mc , *Fonsatti E, Nicolay HJ, Altomonte M, Covre A, Maio M: Targeting cancer vasculature via endoglin/CD105: a novel antibody-based diagnostic and therapeutic strategy in solid tumours. Cardiovasc Res. 2010 Apr 1;86 (1) :12-9. doi: 10.1093/cvr/cvp332., URL: http://www.ncbi.nlm.nih.gov/pubmed/19812043

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The rat monoclonal antibody MJ7/18 reacts with an extracellular epitope of CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-5 μg/ml.

• Isotype:

  Rat IgG2a kappa