Anti-Ms CD44 Biotin

Anti-Ms CD44 Biotin

• Background: CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
• Specifications: The rat monoclonal antibody IM7 reacts with an extracellular epitope of CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.) it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Rat
• Species Reactivity: Dog, Mouse, Human, Cat, Horse
• Immunogen: Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1
• Target Antigen: CD44
• Isotype: IgG2b κ
• Clone: IM7
• Applications: Flow cytometry: Recommended dilution: 1 μg/mL positive control: murine peripheral blood.
• Validated Applications: FC, IP, WB, IHC-P, IHC-F, ICC
• Concentration: 1 mg/mL
• Format: Biotin
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Lesley J, Trowbridge IS: Genetic characterization of a polymorphic murine cell-surface glycoprotein. Immunogenetics. 1982 Mar15(3):313-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/7068175?itool=,*Hegde VL, Singh NP, Nagarkatti PS, Nagarkatti M: CD44 mobilization in allogeneic dendritic cell-T cell immunological synapse plays a key role in T cell activation. J Leukoc Biol. 2008 Jul84(1):134-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/18388297?itool,*van Royen N, Voskuil M, Hoefer I, Jost M, de Graaf S, Hedwig F, Andert JP, Wormhoudt TA, Hua J, Hartmann S, Bode C, Buschmann I, Schaper W, van der Neut R, Piek JJ, Pals ST: CD44 regulates arteriogenesis in mice and is differentially expressed in patients with poor and good collateralization. Circulation. 2004 Apr 6109(13):1647-52. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15023889?itool,*McKallip RJ, Do Y, Fisher MT, Robertson JL, Nagarkatti PS, Nagarkatti M: Role of CD44 in activation-induced cell death: CD44-deficient mice exhibit enhanced T cell response to conventional and superantigens. Int Immunol. 2002 Sep14(9):1015-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/12202399?itool,*Katoh S, Matsumoto N, Kawakita K, Tominaga A, Kincade PW, Matsukura S: A role for CD44 in an antigen-induced murine model of pulmonary eosinophilia. J Clin Invest. 2003 May111(10):1563-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/12750406?itool,*Legg JW, Lewis CA, Parsons M, Ng T, Isacke CM: A novel PKC-regulated mechanism controls CD44 ezrin association and directional cell motility. Nat Cell Biol. 2002 Jun4(6):399-407., URL: https://www.ncbi.nlm.nih.gov/pubmed/12032545?itool,*Si-Tahar M, Sitaraman S, Shibahara T, Madara JL: Negative regulation of epithelium-neutrophil interactions via activation of CD44. Am J Physiol Cell Physiol. 2001 Mar280(3):C423-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/11171560?itool,*Larkin J, Renukaradhya GJ, Sriram V, Du W, Gervay-Hague J, Brutkiewicz RR: CD44 differentially activates mouse NK T cells and conventional T cells. J Immunol. 2006 Jul 1177(1):268-79., URL: https://www.ncbi.nlm.nih.gov/pubmed/16785522?itool,*Xu H, Manivannan A, Liversidge J, Sharp PF, Forrester JV, Crane IJ: Involvement of CD44 in leukocyte trafficking at the blood-retinal barrier. J Leukoc Biol. 2002 Dec72(6):1133-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/12488494?itool,*Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug20(8):1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/,
• Storage Conditions: Store at 2-8°C. Do not freeze.