Anti-Ms CD16/CD32 PE

• Description:

  CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Unlike human, the murine protein is expressed only as a transmembrane isoform. Also CD32 (FcgammaRII) is a low affinity receptor for IgG, but its affinity is lower than that of CD16. These receptors are expressed on monocytes/macrophages, NK cells, granulocytes, mast cells, dendritic cells, and B cells. Their role is to mediate adaptive immune responses through binding the antibody-antigen immunocomplexes, but their effect on the particular cell differs according to the cell type.

• Certification:

  RUO

• Immunogen:

  Murine pre-B cells

• Target Antigen:

  CD16/CD32

• Clone:

  93

• Applications:

  FC (QC tested)

• Concentration:

  0.5 mg/mL

• Format:

  PE

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Oliver AM, Martin F, Kearney JF: Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells. J Immunol. 1997 Feb 1;158 (3) :1108-15., URL: http://www.ncbi.nlm.nih.gov/pubmed/9013949 , *Welch JS, Yuan W, Ley TJ: PML-RARA can increase hematopoietic self-renewal without causing a myeloproliferative disease in mice. J Clin Invest. 2011 Apr;121 (4) :1636-45. doi: 10.1172/JCI42953., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=welch+js+yuan+w , *Somervaille TC, Cleary ML: Identification and characterization of leukemia stem cells in murine MLL-AF9 acute myeloid leukemia. Cancer Cell. 2006 Oct;10 (4) :257-68., URL: http://www.ncbi.nlm.nih.gov/pubmed/17045204 , *Clémenceau B, Vivien R, Pellat C, Foss M, Thibault G, Vié H: The human natural killer cytotoxic cell line NK-92, once armed with a murine CD16 receptor, represents a convenient cellular tool for the screening of mouse mAbs according to their ADCC potential. MAbs. 2013 Jul-Aug;5 (4) :587-94. doi: 10.4161/mabs.25077., URL: http://www.ncbi.nlm.nih.gov/pubmed/23770975 , *Siggs OM, Li X, Xia Y, Beutler B: ZBTB1 is a determinant of lymphoid development. J Exp Med. 2012 Jan 16;209 (1) :19-27., URL: http://www.ncbi.nlm.nih.gov/pubmed/22201126 , *Böiers C, Buza-Vidas N, Jensen CT, Pronk CJ, Kharazi S, Wittmann L, Sitnicka E, Hultquist A, Jacobsen SE: Expression and role of FLT3 in regulation of the earliest stage of normal granulocyte-monocyte progenitor development. Blood. 2010 Jun 17;115 (24) :5061-8. doi: 10.1182/blood-2009-12-258756., URL: http://www.ncbi.nlm.nih.gov/pubmed/20393130

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The rat monoclonal antibody 93 recognizes a common extracellular epitope of murine CD16 (FcgammaRIII) and CD32 (FcgammaRII), the low affinity receptors for IgG.

• Applications Notes:

  Flow cytometry: Recommended dilution: 2 μg/ml.

• Species:

  Mouse

• CAS Number:

  9007-83-4

• Isotype:

  Rat IgG2a lambda