Anti-Ms CD16/CD32 FITC

Anti-Ms CD16/CD32 FITC

• Background: CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Unlike human, the murine protein is expressed only as a transmembrane isoform. Also CD32 (FcgammaRII) is a low affinity receptor for IgG, but its affinity is lower than that of CD16. These receptors are expressed on monocytes/macrophages, NK cells, granulocytes, mast cells, dendritic cells, and B cells. Their role is to mediate adaptive immune responses through binding the antibody-antigen immunocomplexes, but their effect on the particular cell differs according to the cell type.
• Specifications: The rat monoclonal antibody 93 recognizes a common extracellular epitope of murine CD16 (FcgammaRIII) and CD32 (FcgammaRII), the low affinity receptors for IgG.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Rat
• Species Reactivity: Mouse
• Immunogen: Murine pre-B cells
• Target Antigen: CD16/CD32
• Isotype: IgG2a λ
• Clone: 93
• Applications: Flow cytometry: Recommended dilution: 2-5 μg/mL.
• Validated Applications: FC
• Concentration: 0.5 mg/mL
• Format: FITC
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Oliver AM, Martin F, Kearney JF: Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells. J Immunol. 1997 Feb 1158(3):1108-15., URL: http://www.ncbi.nlm.nih.gov/pubmed/9013949 ,*Welch JS, Yuan W, Ley TJ: PML-RARA can increase hematopoietic self-renewal without causing a myeloproliferative disease in mice. J Clin Invest. 2011 Apr121(4):1636-45. doi: 10.1172/JCI42953., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=welch+js+yuan+w ,*Somervaille TC, Cleary ML: Identification and characterization of leukemia stem cells in murine MLL-AF9 acute myeloid leukemia. Cancer Cell. 2006 Oct10(4):257-68., URL: http://www.ncbi.nlm.nih.gov/pubmed/17045204 ,*Clémenceau B, Vivien R, Pellat C, Foss M, Thibault G, Vié H: The human natural killer cytotoxic cell line NK-92, once armed with a murine CD16 receptor, represents a convenient cellular tool for the screening of mouse mAbs according to their ADCC potential. MAbs. 2013 Jul-Aug5(4):587-94. doi: 10.4161/mabs.25077., URL: http://www.ncbi.nlm.nih.gov/pubmed/23770975 ,*Siggs OM, Li X, Xia Y, Beutler B: ZBTB1 is a determinant of lymphoid development. J Exp Med. 2012 Jan 16209(1):19-27., URL: http://www.ncbi.nlm.nih.gov/pubmed/22201126 ,*Böiers C, Buza-Vidas N, Jensen CT, Pronk CJ, Kharazi S, Wittmann L, Sitnicka E, Hultquist A, Jacobsen SE: Expression and role of FLT3 in regulation of the earliest stage of normal granulocyte-monocyte progenitor development. Blood. 2010 Jun 17115(24):5061-8. doi: 10.1182/blood-2009-12-258756., URL: http://www.ncbi.nlm.nih.gov/pubmed/20393130,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.