Anti-Rt CD161 FITC

Anti-Rt CD161 FITC

• Background: CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.
• Specifications: The mouse monoclonal antibody 10/78 recognizes CD161, an approximately 30 kDa type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer. A common extracellular epitope on rat CD161a and b isoforms is detected.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Rat
• Immunogen: Splenic cells purified from the LEW rat
• Target Antigen: CD161
• Isotype: Igg1 κ
• Clone: 10/78
• Applications: Flow cytometry: Recommended dilution: 2-5 μg/mL.
• Validated Applications: FC
• Concentration: 0.5 mg/mL
• Format: FITC
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Tliba O, Chauvin A, Le Vern Y, Boulard C, Sbille P: Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats. Vet Res. 2002 May-Jun33(3):327-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/12056483,*Sedgwick JD, Ford AL, Foulcher E, Airriess R: Central nervous system microglial cell activation and proliferation follows direct interaction with tissue-infiltrating T cell blasts. J Immunol. 1998 Jun 1160(11):5320-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/9605131,*Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov26(11):2582-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/8921942,*May E, Dorris ML, Satumtira N, Iqbal I, Rehman MI, Lightfoot E, Taurog JD: CD8 alpha beta T cells are not essential to the pathogenesis of arthritis or colitis in HLA-B27 transgenic rats. J Immunol. 2003 Jan 15170(2):1099-105., URL: https://www.ncbi.nlm.nih.gov/pubmed/12517979,*Teshima R, Nakamura R, Nakamura R, Hachisuka A, Sawada JI, Shibutani M: Effects of exposure to decabromodiphenyl ether on the development of the immune system in rats. J Health Sci 200854(4):382-389, URL: ,*Chang CJ, Tai KF, Roffler S, Hwang LH: The immunization site of cytokine-secreting tumor cell vaccines influences the trafficking of tumor-specific T lymphocytes and antitumor efficacy against regional tumors. J Immunol. 2004 Nov 15173(10):6025-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/15528337,*Stephens LA, Barclay AN, Mason D: Phenotypic characterization of regulatory CD4+CD25+ T cells in rats. Int Immunol. 2004 Feb16(2):365-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/14734622,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.