Anti-Rt CD161 FITC

• Description:

  CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.

• Certification:

  RUO

• Reactivity:

  Rat

• Immunogen:

  Splenic cells purified from the LEW rat

• Target Antigen:

  CD161

• Clone:

  10/78

• Applications:

  FC (QC tested)

• Concentration:

  0.5 mg/mL

• Format:

  FITC

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Tliba O, Chauvin A, Le Vern Y, Boulard C, Sbille P: Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats. Vet Res. 2002 May-Jun;33 (3) :327-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/12056483, *Sedgwick JD, Ford AL, Foulcher E, Airriess R: Central nervous system microglial cell activation and proliferation follows direct interaction with tissue-infiltrating T cell blasts. J Immunol. 1998 Jun 1;160 (11) :5320-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/9605131, *Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov;26 (11) :2582-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/8921942, *May E, Dorris ML, Satumtira N, Iqbal I, Rehman MI, Lightfoot E, Taurog JD: CD8 alpha beta T cells are not essential to the pathogenesis of arthritis or colitis in HLA-B27 transgenic rats. J Immunol. 2003 Jan 15;170 (2) :1099-105., URL: https://www.ncbi.nlm.nih.gov/pubmed/12517979, *Teshima R, Nakamura R, Nakamura R, Hachisuka A, Sawada JI, Shibutani M: Effects of exposure to decabromodiphenyl ether on the development of the immune system in rats. J Health Sci 2008;54 (4) :382-389, URL: , *Chang CJ, Tai KF, Roffler S, Hwang LH: The immunization site of cytokine-secreting tumor cell vaccines influences the trafficking of tumor-specific T lymphocytes and antitumor efficacy against regional tumors. J Immunol. 2004 Nov 15;173 (10) :6025-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/15528337, *Stephens LA, Barclay AN, Mason D: Phenotypic characterization of regulatory CD4+CD25+ T cells in rats. Int Immunol. 2004 Feb;16 (2) :365-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/14734622

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 10/78 recognizes CD161, an approximately 30 kDa type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer. A common extracellular epitope on rat CD161a and b isoforms is detected.

• Applications Notes:

  Flow cytometry: Recommended dilution: 2-5 μg/ml.

• Isotype:

  Mouse IgG1 kappa