Anti-Rt CD8b FITC

• Description:

  The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

• Certification:

  RUO

• Reactivity:

  Rat

• Immunogen:

  CD8 positive Wistar rat splenic T cell hybridomas

• Target Antigen:

  CD8b

• Clone:

  341

• Applications:

  FC (QC tested)

• Concentration:

  0.5 mg/mL

• Format:

  FITC

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov;22 (11) :2841-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1358623, *Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov;26 (11) :2582-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/8921942, *Nohara O, Kulka M, Déry RE, Wills FL, Hirji NS, Gilchrist M, Befus AD: Regulation of CD8 expression in mast cells by exogenous or endogenous nitric oxide. J Immunol. 2001 Nov 15;167 (10) :5935-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/11698471, *Ellerman KE, Like AA: Islet cell membrane antigens activate diabetogenic CD4+ T-cells in the BB/Wor rat. Diabetes. 1999 May;48 (5) :975-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/10331400, *Lin TJ, Hirji N, Nohara O, Stenton GR, Gilchrist M, Befus AD: Mast cells express novel CD8 molecules that selectively modulate mediator secretion. J Immunol. 1998 Dec 1;161 (11) :6265-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/9834115, *Hirji N, Lin TJ, Bissonnette E, Belosevic M, Befus AD: Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J Immunol. 1998 Jun 15;160 (12) :6004-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/9637515, *Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15;158 (4) :1833-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/9029123, *Kühnlein P, Park JH, Herrmann T, Elbe A, Hünig T: Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J Immunol. 1994 Aug 1;153 (3) :979-86., URL: https://www.ncbi.nlm.nih.gov/pubmed/8027567, *Mabarrack NH, Turner NL, Mayrhofer G: Recent thymic origin, differentiation, and turnover of regulatory T cells. J Leukoc Biol. 2008 Nov;84 (5) :1287-97., URL: https://www.ncbi.nlm.nih.gov/pubmed/18682578

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-4 μg/ml.

• Isotype:

  Mouse IgG1 kappa