Anti-Rt CD28 FITC

Anti-Rt CD28 FITC

• Background: CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
• Specifications: The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Rat
• Immunogen: Mouse A20J B lymphoma cells transfected with rat CD28
• Target Antigen: CD28
• Isotype: Igg1 κ
• Clone: JJ319
• Applications: Flow cytometry: Recommended dilution: 2 μg/mL.
• Validated Applications: FC
• Concentration: 0.5 mg/mL
• Format: FITC
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Haspot F, Villemain F, Laflamme G, Coulon F, Olive D, Tiollier J, Soulillou JP, Vanhove B: Differential effect of CD28 versus B7 blockade on direct pathway of allorecognition and self-restricted responses. Blood. 2002 Mar 1599(6):2228-34., URL: http://www.ncbi.nlm.nih.gov/pubmed/11877302 ,*Laskowski IA, Pratschke J, Wilhelm MJ, Dong VM, Beato F, Taal M, Gasser M, Hancock WW, Sayegh MH, Tilney NL: Anti-CD28 monoclonal antibody therapy prevents chronic rejection of renal allografts in rats. J Am Soc Nephrol. 2002 Feb13(2):519-27., URL: http://www.ncbi.nlm.nih.gov/pubmed/11805183 ,*van den Brandt J, Wang D, Reichardt HM: Resistance of single-positive thymocytes to glucocorticoid-induced apoptosis is mediated by CD28 signaling. Mol Endocrinol. 2004 Mar18(3):687-95., URL: http://www.ncbi.nlm.nih.gov/pubmed/14701943 ,*Kerstan A, Armbruster N, Leverkus M, Hünig T: Cyclosporin A abolishes CD28-mediated resistance to CD95-induced apoptosis via superinduction of caspase-3. J Immunol. 2006 Dec 1177(11):7689-97., URL: http://www.ncbi.nlm.nih.gov/pubmed/17114439 ,*Guillonneau C, Séveno C, Dugast AS, Li XL, Renaudin K, Haspot F, Usal C, Veziers J, Anegon I, Vanhove B: Anti-CD28 antibodies modify regulatory mechanisms and reinforce tolerance in CD40Ig-treated heart allograft recipients. J Immunol. 2007 Dec 15179(12):8164-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/18056359 ,*Dengler TJ, Szabo G, Sido B, Nottmeyer W, Zimmerman R, Vahl CF, Hünig T, Meuer SC: Prolonged allograft survival but no tolerance induction by modulating CD28 antibody JJ319 after high-responder rat heart transplantation. Transplantation. 1999 Feb 1567(3):392-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/10030284 ,*Lühder F, Huang Y, Dennehy KM, Guntermann C, Müller I, Winkler E, Kerkau T, Ikemizu S, Davis SJ, Hanke T, Hünig T: Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists. J Exp Med. 2003 Apr 21197(8):955-66., URL: http://www.ncbi.nlm.nih.gov/pubmed/12707299 ,*Tacke M, Hanke G, Hanke T, Hünig T: CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28. Eur J Immunol. 1997 Jan27(1):239-47., URL: https://www.ncbi.nlm.nih.gov/pubmed/9022025,*Robinson LE, Clandinin MT, Field CJ: R3230AC rat mammary tumor and dietary long-chain (n-3) fatty acids change immune cell composition and function during mitogen activation. J Nutr. 2001 Jul131(7):2021-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/11435524,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.