Anti-Ms CD18 FITC

• Description:

  CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18) . In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

• Certification:

  RUO

• Negative Species:

  Dog

• Reactivity:

  Mouse

• Immunogen:

  Murine cytotoxic T cell glycoproteins

• Target Antigen:

  CD18

• Clone:

  M18/2

• Applications:

  FC (QC tested)

• Concentration:

  0.5 mg/mL

• Format:

  FITC

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Cullere X, Lauterbach M, Tsuboi N, Mayadas TN: Neutrophil-selective CD18 silencing using RNA interference in vivo. Blood. 2008 Apr 1;111 (7) :3591-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/18216298?itool, *Abraham C, Miller J: Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1. J Immunol. 2001 Nov 1;167 (9) :5193-201., URL: https://www.ncbi.nlm.nih.gov/pubmed/11673532?itool, *Barlow SC, Langston W, Matthews KM, Chidlow JH Jr, Kevil CG: CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes. Am J Pathol. 2004 Dec;165 (6) :1849-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/15579429?itool, *Varga G, Balkow S, Wild MK, Stadtbaeumer A, Krummen M, Rothoeft T, Higuchi T, Beissert S, Wethmar K, Scharffetter-Kochanek K, Vestweber D, Grabbe S: Active MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation. Blood. 2007 Jan 15;109 (2) :661-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/17003381?itool, *Sanchez-Madrid F, Simon P, Thompson S, Springer TA: Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983 Aug 1;158 (2) :586-602., URL: https://www.ncbi.nlm.nih.gov/pubmed/6193226?itool=, *Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18) . J Immunol. 1993 May 15;150 (10) :4466-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/8097756?itool=, *Zahálka MA, Naor D: Beta 2-integrin dependent aggregate formation between LB T cell lymphoma and spleen cells: assessment of correlation with spleen invasiveness. Int Immunol. 1994 Jun;6 (6) :917-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/7522049?itool=, *Watts GM, Beurskens FJ, Martin Padura, Ballantyne CM, Klickstein LB, Brenner MB, Lee DM: Manifestations of inflammatory arthritis are critically dependent on LFA-1. J Immunol. 2005 Mar 15;174 (6) :3668-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/15749905?itool, *Avni O, Pur Z, Yefenof E, Baniyash M: Complement receptor 3 of macrophages is associated with galectin-1-like protein. J Immunol. 1998 Jun 15;160 (12) :6151-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9637533?itool=, *Sakurai E, Taguchi H, Anand A, Ambati BK, Gragoudas ES, Miller JW, Adamis AP, Ambati J: Targeted disruption of the CD18 or ICAM-1 gene inhibits choroidal neovascularization. Invest Ophthalmol Vis Sci. 2003 Jun;44 (6) :2743-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/12766082?itool

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The rat monoclonal antibody M18/2 recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-2 μg/ml.

• Isotype:

  Rat IgG2a