Anti-Hu Ig Lambda Light Chain FITC

• Background:

  Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

• Specifications:

  The antibody 4C2 reacts with lambda light chains (22.5 kDa) of human immunoglobulin.

• CAS Number:

  9007-83-4

• Certification:

  RUO

• Host:

  Mouse

• Species Reactivity:

  Human

• Negative Species:

  Rabbit, Hamster, Sheep, Guinea pig, Goat

• Target Antigen:

  Ig Lambda Light Chain

• Isotype:

  IgG1

• Clone:

  4C2

• Applications:

  Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

• Validated Applications:

  FC

• Format:

  FITC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Os A, Bürgler S, Ribes AP, Funderud A, Wang D, Thompson KM, Tj248nnfjord GE, Bogen B, Munthe LA: Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells. Cell Rep. 2013 Aug 154(3):566-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/23933259,

• Other References:

  *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690?ordinalp,

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.