Anti-Hu CD133 APC

Anti-Hu CD133 APC

• Background: CD133 (prominin 1) is a 5-transmembrane glycoprotein with extracellular N- and intracellular C-terminus. CD133 function remains to be elucidated, but it can be used as a cancer stem cell marker. Its expression pattern in progenitor cells is similar to CD34, i.e. on hematopoietic stem cells in bone marrow, cord blood, neural stem cells, retinoblastoma, or endothelial precursor cells (not mature endothelial cells). It is being used for identification and isolation of hematopoietic stem cells, including isolation for stem cell transplantation. Expression of CD133 correlates with differentiation of human colon cancer cells.
• Specifications: The mouse monoclonal antibody W6B3C1 recognizes the extracellular glycosylated epitope 1 on human CD133 (CD133/1), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of stem cells and tumor cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: WERI-RB-1 retinoblastoma cell line
• Target Antigen: CD133
• Isotype: IgG1
• Clone: W6B3C1
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: APC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Liu C, Li Y, Xing Y, Cao B, Yang F, Yang T, Ai Z, Wei Y, Jiang J: The Interaction between Cancer Stem Cell Marker CD133 and Src Protein Promotes Focal Adhesion Kinase (FAK) Phosphorylation and Cell Migration. J Biol Chem. 2016 Jul 22291(30):15540-50. , URL: https://www.ncbi.nlm.nih.gov/pubmed/27226554,*Rappa G, Fodstad O, Lorico A: The stem cell-associated antigen CD133 (Prominin-1) is a molecular therapeutic target for metastatic melanoma. Stem Cells. 2008 Dec26(12):3008-17. , URL: https://www.ncbi.nlm.nih.gov/pubmed/18802032 ,*Hermansen SK, Christensen KG, Jensen SS, Kristensen BW: Inconsistent immunohistochemical expression patterns of four different CD133 antibody clones in glioblastoma. J Histochem Cytochem. 2011 Apr59(4):391-407., URL: https://www.ncbi.nlm.nih.gov/pubmed/21411810 ,*Feng HL, Liu YQ, Yang LJ, Bian XC, Yang ZL, Gu B, Zhang H, Wang CJ, Su XL, Zhao XM: Expression of CD133 correlates with differentiation of human colon cancer cells. Cancer Biol Ther. 2010 Feb9(3):216-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=feng+hl+yang+zl+zhang+h ,*Dahlrot RH, Hermansen SK, Hansen S, Kristensen BW: What is the clinical value of cancer stem cell markers in gliomas? Int J Clin Exp Pathol. 20136(3):334-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/23412423 ,*Brescia P, Ortensi B, Fornasari L, Levi D, Broggi G, Pelicci G: CD133 is essential for glioblastoma stem cell maintenance. Stem Cells. 2013 May31(5):857-69. , URL: https://www.ncbi.nlm.nih.gov/pubmed/23307586 ,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.