Anti-Hu CD103 APC

CD103 / integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain) . It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.

Human, Non-human primates

HTLV-1 induced human T cell line MAPS16

CD103

Ber-ACT8

FC (QC tested)

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Poles MA, Barsoum S, Yu W, Yu J, Sun P, Daly J, He T, Mehandru S, Talal A, Markowitz M, Hurley A, Ho D, Zhang L: Human immunodeficiency virus type 1 induces persistent changes in mucosal and blood gammadelta T cells despite suppressive therapy. J Virol. 2003 Oct;77 (19) :10456-67., URL: http://www.ncbi.nlm.nih.gov/pubmed/12970431 , *Jaensson E, Uronen-Hansson H, Pabst O, Eksteen B, Tian J, Coombes JL, Berg PL, Davidsson T, Powrie F, Johansson-Lindbom B, Agace WW: Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans. J Exp Med. 2008 Sep 1;205 (9) :2139-49., URL: https://www.ncbi.nlm.nih.gov/pubmed/18710932, *Zhang L, Bertucci AM, Ramsey-Goldman R, Burt RK, Datta SK: Regulatory T cell (Treg) subsets return in patients with refractory lupus following stem cell transplantation, and TGF-beta-producing CD8+ Treg cells are associated with immunological remission of lupus. J Immunol. 2009 Nov 15;183 (10) :6346-58., URL: https://www.ncbi.nlm.nih.gov/pubmed/19841178, *Cardoso CR, Garlet GP, Moreira AP, Júnior WM, Rossi MA, Silva JS: Characterization of CD4+CD25+ natural regulatory T cells in the inflammatory infiltrate of human chronic periodontitis. J Leukoc Biol. 2008 Jul;84 (1) :311-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/18451325, *Brandes M, Willimann K, Lang AB, Nam KH, Jin C, Brenner MB, Morita CT, Moser B: Flexible migration program regulates gamma delta T-cell involvement in humoral immunity. Blood. 2003 Nov 15;102 (10) :3693-701., URL: https://www.ncbi.nlm.nih.gov/pubmed/12881309, *Kost CB, Holden JT, Mann KP: Marginal zone B-cell lymphoma: a retrospective immunophenotypic analysis. Cytometry B Clin Cytom. 2008 Sep;74 (5) :282-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/18500740, *French JJ, Cresswell J, Wong WK, Seymour K, Charnley RM, Kirby JA: T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy? Br J Cancer. 2002 Oct 21;87 (9) :1034-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/12434297, *Sigmundsdottir H, Johnston A, Gudjonsson JE, Bjarnason B, Valdimarsson H: Methotrexate markedly reduces the expression of vascular E-selectin, cutaneous lymphocyte-associated antigen and the numbers of mononuclear leucocytes in psoriatic skin. Exp Dermatol. 2004 Jul;13 (7) :426-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/15217363, *León F, Roy G: Isolation of human small bowel intraepithelial lymphocytes by annexin V-coated magnetic beads. Lab Invest. 2004 Jun;84 (6) :804-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/15154017

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes.

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