Anti-Hu CD73 APC

Anti-Hu CD73 APC

• Background: CD73 (ecto-5´-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein.
• Specifications: The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells and endothelial cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Non-human primates
• Immunogen: pre-B leukemia cells
• Target Antigen: CD73
• Isotype: Igg1 κ
• Clone: AD2
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: APC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 279:852., URL: https://www.ncbi.nlm.nih.gov/pubmed/29780382 ,*Paquet-Fifield S, Schlüter H, Li A, Aitken T, Gangatirkar P, Blashki D, Koelmeyer R, Pouliot N, Palatsides M, Ellis S, Brouard N, Zannettino A, Saunders N, Thompson N, Li J, Kaur P: A role for pericytes as microenvironmental regulators of human skin tissue regeneration. J Clin Invest. 2009 Sep119(9):2795-806., URL: https://pubmed.ncbi.nlm.nih.gov/19652362/,*Hashikawa T, Takedachi M, Terakura M, Saho T, Yamada S, Thompson LF, Shimabukuro Y, Murakami S: Involvement of CD73 (ecto-5'-nucleotidase) in adenosine generation by human gingival fibroblasts. J Dent Res. 2003 Nov82(11):888-92., URL: http://www.ncbi.nlm.nih.gov/pubmed/14578500 ,*Semenov OV, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch AH, Malek A: Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation. Am J Obstet Gynecol. 2010 Feb202(2):193, URL: http://www.ncbi.nlm.nih.gov/pubmed/20035913 ,*Nemoto E, Tada H, Shimauchi H: Disruption of CD40/CD40 ligand interaction with cleavage of CD40 on human gingival fibroblasts by human leukocyte elastase resulting in down-regulation of chemokine production. J Leukoc Biol. 2002 Sep72(3):538-45., URL: https://www.ncbi.nlm.nih.gov/pubmed/12223522,*Mokrý J, Soukup T, Mičuda S, Karbanová J, Víšek B, Brčáková E, Suchánek J, Bouchal J, Vokurková D, Ivančáková R: Telomere attrition occurs during ex vivo expansion of human dental pulp stem cells. J Biomed Biotechnol. 20102010:673513., URL: https://www.ncbi.nlm.nih.gov/pubmed/20976265,*Kögler G, Sensken S, Airey JA, Trapp T, Müschen M, Feldhahn N, Liedtke S, Sorg RV, Fischer J, Rosenbaum C, Greschat S, Knipper A, Bender J, Degistirici O, Gao J, Caplan AI, Colletti EJ, Almeida-Porada G, Müller HW, Zanjani E, Wernet P: A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential. J Exp Med. 2004 Jul 19200(2):123-35., URL: https://pubmed.ncbi.nlm.nih.gov/15263023/,*Rodriguez MW, Paquet AC, Yang YH, Erle DJ: Differential gene expression by integrin beta 7+ and beta 7- memory T helper cells. BMC Immunol. 2004 Jul 55:13., URL: https://www.ncbi.nlm.nih.gov/pubmed/15236665,*Borrione P, Peola S, Mariani S, Besostri B, Mallone R, Malavasi F, Pileri A, Massaia M: CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3-kinase pathway and inducing CD73 expression. J Immunol. 1999 May 15162(10):6238-46., URL: https://www.ncbi.nlm.nih.gov/pubmed/10229870,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.