Anti-Hu CD70 APC

• Description:

  CD70, also known as TNFSF7 or CD27L, is a 50 kDa type II transmembrane glycoprotein of the TNF superfamily. It is expressed mainly on activated lymphocytes, including NK cells, and forms trimeric structure. CD70 plays a role in T-cell activation, proliferation and differentiation, in enhancing the generation of cytolytic T cells, and in long-term maintenance of T cell memory. It is also involved in B cell differentiation induced by activated plasmacytoid dendritic cells, which also express CD70.

• Certification:

  RUO

• Immunogen:

  Human L428 cell line

• Target Antigen:

  CD70

• Clone:

  Ki-24

• Applications:

  FC (QC tested)

• Format:

  APC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Hashimoto-Okada M, Kitawaki T, Kadowaki N, Iwata S, Morimoto C, Hori T, Uchiyama T: The CD70-CD27 interaction during the stimulation with dendritic cells promotes naive CD4 (+) T cells to develop into T cells producing a broad array of immunostimulatory cytokines in humans. Int Immunol. 2009 Aug;21 (8) :891-904., URL: http://www.ncbi.nlm.nih.gov/pubmed/19556308 Kaufma, *Israel BF, Pickles RJ, Segal DM, Gerard RD, Kenney SC: Enhancement of adenovirus vector entry into CD70-positive B-cell lines by using a bispecific CD70-adenovirus fiber antibody. J Virol. 2001 Jun;75 (11) :5215-21., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=israel+bf+pickles+rj , *Trebing J, El-Mesery M, Schäfer V, Weisenberger D, Siegmund D, Silence K, Wajant H. CD70-restricted specific activation of TRAILR1 or TRAILR2 using scFv-targeted TRAIL mutants. Cell Death Dis. 2014 Jan 30;5:e1035., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=trebing+j+weisenberger+d+silence+k , *Kaufmann Y, Amariglio N, Rosenthal E, Hirsch YJ, Many A, Rechavi G: Proliferation response of leukemic cells to CD70 ligation oscillates with recurrent remission and relapse in a low-grade lymphoma. J Immunol. 2005 Nov 15;175 (10) :6940-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=kaufmann+y+amariglio+n

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody Ki-24 recognizes an extracellular epitope of CD70, an approximately 50 kDa type II transmembrane glycoprotein expressed on activated lymphocytes and some B cell leukemias.

• Applications Notes:

  6

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG3 kappa