Anti-Hu CD271 APC

Anti-Hu CD271 APC

• Background: CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.
• Specifications: The mouse monoclonal antibody NGFR5 (originally C34C) recognizes an epitope within ammino acids 1 - 160 of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Rabbit, Cat, Non-human primates, Ferret
• Negative Species: Mouse, Rat
• Immunogen: Purified CD271 protein isolated from human melanoma cell line A875
• Target Antigen: CD271
• Isotype: IgG1
• Clone: NGFR5
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests. Extracellular and intracellular staining.
• Validated Applications: FC
• Format: APC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Thompson SJ, Schatteman GC, Gown AM, Bothwell M: A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue. Am J Clin Pathol. 1989 Oct92(4):415-23., URL: http://www.ncbi.nlm.nih.gov/pubmed/2552791,*Marano N, Dietzschold B, Earley JJ Jr, Schatteman G, Thompson S, Grob P, Ross AH, Bothwell M, Atkinson BF, Koprowski H: Purification and amino terminal sequencing of human melanoma nerve growth factor receptor. J Neurochem. 1987 Jan48(1):225-32., URL: http://www.ncbi.nlm.nih.gov/pubmed/3025363 ,*Schatteman GC, Gibbs L, Lanahan AA, Claude P, Bothwell M: Expression of NGF receptor in the developing and adult primate central nervous system. J Neurosci. 1988 Mar8(3):860-73., URL: http://www.ncbi.nlm.nih.gov/pubmed/2831315 ,*Schatteman GC, Gibbs L, Lanahan AA, Claude P, Bothwell M: Expression of NGF receptor in the developing and adult primate central nervous system. J Neurosci. 1988 Mar8(3):860-73., URL: http://www.ncbi.nlm.nih.gov/pubmed/2831315 ,*Alpers CE, Hudkins KL, Ferguson M, Johnson RJ, Schatteman GC, Bothwell M: Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys. Lab Invest. 1993 Dec69(6):703-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/7903404 ,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.