Anti-Hu CD271 APC

• Description:

  CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.

• Certification:

  RUO

• Negative Species:

  Mouse, Rat

• Reactivity:

  Human, Rabbit, Cat, Non-human primates, Ferret

• Immunogen:

  Purified CD271 protein isolated from human melanoma cell line A875

• Target Antigen:

  CD271

• Clone:

  NGFR5

• Applications:

  FC (QC tested), FC-IC (verified)

• Format:

  APC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Thompson SJ, Schatteman GC, Gown AM, Bothwell M: A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue. Am J Clin Pathol. 1989 Oct;92 (4) :415-23., URL: http://www.ncbi.nlm.nih.gov/pubmed/2552791, *Marano N, Dietzschold B, Earley JJ Jr, Schatteman G, Thompson S, Grob P, Ross AH, Bothwell M, Atkinson BF, Koprowski H: Purification and amino terminal sequencing of human melanoma nerve growth factor receptor. J Neurochem. 1987 Jan;48 (1) :225-32., URL: http://www.ncbi.nlm.nih.gov/pubmed/3025363 , *Schatteman GC, Gibbs L, Lanahan AA, Claude P, Bothwell M: Expression of NGF receptor in the developing and adult primate central nervous system. J Neurosci. 1988 Mar;8 (3) :860-73., URL: http://www.ncbi.nlm.nih.gov/pubmed/2831315 , *Alpers CE, Hudkins KL, Ferguson M, Johnson RJ, Schatteman GC, Bothwell M: Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys. Lab Invest. 1993 Dec;69 (6) :703-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/7903404 , *Wong ST, Henley JR, Kanning KC, Huang K, Bothwell M, Poo M: A p75 (NTR) and Nogo receptor complex mediates repulsive signaling by myelin-associated glycoprotein. Nat Neurosci. 2002 Dec;5 (12) :1302-8. (IP, WB, ICC), URL: https://pubmed.ncbi.nlm.nih.gov/12426574/, *Maeda K, Matsuda M, Suzuki H, Saitoh HA: Immunohistochemical Recognition of Human Follicular Dendritic Cells (FDCs) in Routinely Processed Paraffin Sections. J Histochem Cytochem. 2002 Nov;50 (11) :1475-86. (IHC-P), URL: https://pubmed.ncbi.nlm.nih.gov/12417613/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody NGFR5 (originally C34C) recognizes an epitope within ammino acids 1 - 160 of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.

• Applications Notes:

  6

• Isotype:

  Mouse IgG1